Cryptococcal Yeast Cells Invade the Central Nervous System via Transcellular Penetration of the Blood-Brain Barrier

Re-Joining the Publications journey from Johns Hopkins after a decade.

Dr. Kim’s lab was having problems as the Transcytosis, Invasion and Association assays were not working as expected (in 2003). After I joined the lab, we sorted out most of it and made it functional. One day Dr. Kim called me to his Office and asked me if I can work on Cryptococcus neoformans. I had previously isolated it from patients suffering with AIDS during my consulting days; I said okay and teamed up with Tapan Dam (he is no more now; and was a very kind friend) for this assay. We spent several nights in the lab to complete the experiment by repetition. Finally we succeeded and participated in the data analysis of this paper. This was going to be re-starting of my publications journey after 10 years gap; and being co-author of such an elite team was very exciting.

This work was conducted in Dr. Kwang Sik Kim’s lab in the Division of Pediatrics Infectious Diseases at Johns Hopkins School of Medicine between 2003 to 2005.

Yun C Chang, Monique F Stins, Michael J McCaffery, Georgina F Miller, Dan R Pare, Tapen Dam, Maneesh Paul-Satyaseela, Kwang Sik Kim, Kyung J Kwon-Chung
Infection & Immunity 2004 Sep;72(9):4985–95.

Co-inventor of Enmetazobactam. A microbiologist, techno-commercial enabler, intrapreneur, & new-drug-discovery scientist